Two Mile Creek Conservation Area is a 9 hectare (22 acre) property, owned by the Niagara Peninsula Conservation Authority. 

The Conservation Area protects a significant portion of the Lower Two Mile Creek Valley System, between Lakeshore Road and Niagara Stone Road.  It is a forested floodplain, with significant ecological features.  There are few improvements for public access.  The site is maintained as a natural reserve.

A local trail links Colonel Butler Drive with Butler Street, allowing the residents of Garrison Village to walk into the downtown area.  This walkway is maintained in partnership with the Town of Niagara-on-the-Lake.

! No Horseback riding permitted.